mewo cells bcrc 60540  (BioResource International Inc)

Journal: International Journal of Molecular Sciences

Article Title: Human Microfibrillar-Associated Protein 4 (MFAP4) Gene Promoter: A TATA-Less Promoter That Is Regulated by Retinol and Coenzyme Q10 in Human Fibroblast Cells

doi: 10.3390/ijms21218392

Figure Lengend Snippet: The expression of pEGFP- pMFAP4 plasmids in mouse fibroblast NIH/3T3 cells, human melanoma MeWo cells, and mouse melanoma B16-F10 cells. ( A ) Fluorescence images. Scale bar = 100 μm. ( B ) Relative fluorescence intensity. Data are presented as means ± SE.

Article Snippet: Detroit 551 cells (BCRC 60118, human normal fibroblast cells), MeWo cells (BCRC 60540, human melanoma cells), NIH/3T3 cells (BCRC 60008, mouse fibroblast cells), and B16-F10 cells (BCRC 60031, mouse melanoma cells) were all obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan, ROC).

Techniques: Expressing, Fluorescence

Journal: BioMed Research International

Article Title: Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

doi: 10.1155/2013/732307

Figure Lengend Snippet: Fluorescent images of pEGFP plasmid ((a) to (d)) and pEGFP-CDB ((e) to (h)) plasmid transfected MeWo cells at different time points.

Article Snippet: The human melanoma cell line, MeWo cells (BCRC 60540), was obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan).

Techniques: Plasmid Preparation, Transfection

Journal: BioMed Research International

Article Title: Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

doi: 10.1155/2013/732307

Figure Lengend Snippet: Fluorescent images of pDsRed plasmid ((a) to (d)) and pDsRed-CDB ((e) to (h)) plasmid transfected MeWo cells at different time points.

Article Snippet: The human melanoma cell line, MeWo cells (BCRC 60540), was obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan).

Techniques: Plasmid Preparation, Transfection

Journal: BioMed Research International

Article Title: Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

doi: 10.1155/2013/732307

Figure Lengend Snippet: Relative fluorescent intensities of (a) pEGFP/pEGFP-CDB plasmids and (b) pDsRed/pDsRed-CDB plasmids transfected MeWo cells at different time points. Each value is expressed as mean ± SE ( n = 3).

Article Snippet: The human melanoma cell line, MeWo cells (BCRC 60540), was obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan).

Techniques: Transfection

Journal: BioMed Research International

Article Title: Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

doi: 10.1155/2013/732307

Figure Lengend Snippet: RT-PCR analyses of (a) pEGFP/pEGFP-CDB and (b) pDsRed/pDsRed-CDB plasmids transfected MeWo cells at different time points. Levels of GAPDH mRNA were used as internal control.

Article Snippet: The human melanoma cell line, MeWo cells (BCRC 60540), was obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Transfection, Control

Journal: BioMed Research International

Article Title: Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

doi: 10.1155/2013/732307

Figure Lengend Snippet: (a) Relative fluorescent intensities and (b) induction ratio of curcumin-induced pEGFP/pEGFP-CDB plasmids transfected MeWo cells at different time points.

Article Snippet: The human melanoma cell line, MeWo cells (BCRC 60540), was obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan).

Techniques: Transfection

Journal: BioMed Research International

Article Title: Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

doi: 10.1155/2013/732307

Figure Lengend Snippet: (a) Relative fluorescent intensities and (b) induction ratio of curcumin-induced pDsRed/pDsRed-CDB plasmids transfected MeWo cells at different time points.

Article Snippet: The human melanoma cell line, MeWo cells (BCRC 60540), was obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan).

Techniques: Transfection